F.B. Meyer emphasizes the urgency of God's call, illustrated by the repeated calling of Samuel. He highlights that God's call often grows in intensity and can be discerned through prayer, the counsel of godly friends, and the transformative effects it has on our lives. Meyer encourages believers to act on divine impressions, testing their authenticity by their impact on selflessness and service to others. The sermon underscores the importance of responding quickly and fearlessly to God's call, as it often requires stepping out of comfort zones for His glory.