John Gill emphasizes the importance of the duties of both magistrates and subjects, urging Christians to obey civil authorities as ordained by God to maintain order and prevent scandal to the faith. He explains that subjection is a reasonable service required of all individuals within a commonwealth, regardless of their status, and highlights the responsibilities of magistrates to govern justly and uphold the law. Gill also addresses the need for subjects to honor and respect their rulers, while reminding them that obedience is due only in matters that do not contradict God's laws. The sermon serves as a reminder for Christians to conduct themselves in a manner that reflects the teachings of Christ, thereby dispelling any false accusations against their faith.

John Gill emphasizes the call for believers to live a holy life, urging them to lay aside vices such as malice, guile, hypocrisy, envy, and evil speaking, which are unbecoming of those who are born again. He compares the desire for the Gospel to that of newborn babes craving milk, highlighting its purity and necessity for spiritual growth. Gill encourages believers to embrace their identity as a chosen people, called to offer spiritual sacrifices and to live honorably among others, thereby glorifying God. He also addresses the importance of obedience to civil authorities, reminding them that their freedom in Christ should not be used as a cover for wrongdoing. Ultimately, he points to Christ's example of suffering and righteousness as a model for their conduct.