John Nelson Darby emphasizes the contrast between Christ's divine authority and the rejection He faced from His own people, highlighting the role of the Holy Spirit as the Comforter who guides believers after Christ's ascension. He explains that the Holy Spirit's work involves convicting the world of sin, righteousness, and judgment, while also guiding the saints into all truth. Darby points out that the rejection of Christ reveals the blindness of the human heart and the necessity of recognizing Him as the Son of God. He encourages believers to trust in the Holy Spirit's presence and guidance, reminding them that their identity is rooted in their relationship with Christ. Ultimately, the sermon calls for a deeper understanding of the Holy Spirit's role in revealing the glory of Christ to believers.